Journal: PLOS ONE
Article Title: Blockade of SIRPα-CD47 axis by anti-SIRPα antibody enhances anti-tumor activity of DXd antibody-drug conjugates
doi: 10.1371/journal.pone.0304985
Figure Lengend Snippet: ( A ) The binding of AF488-labeled DS-1103a to human monocyte-derived macrophages was analyzed by flow cytometry. The number of antibodies bound per cell was estimated using the calibration beads and is shown as geomean and geoSD (n = 3). ( B ) Far Red-labeled macrophages were co-cultured with Violet-labeled LK-2 cells and ADCP activity [%] was measured by flow cytometry. The data represent the mean ± standard error (n = 4). P-values were determined by the paired-sample t-test. ***, **, and ns indicate P < 0.001, P < 0.01, and not significant, respectively. Representative data from two independent experiments are shown.
Article Snippet: To quantify DS-1103a binding to macrophages, anti-IgG, Human, Goat-Poly, Microsphere, Quantum Simply Cellular (Bangs Laboratories, Technology Drive Fishers, IN) was used.
Techniques: Binding Assay, Labeling, Derivative Assay, Flow Cytometry, Cell Culture, Activity Assay